Detailed information of TA system TA099353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3117350..3118187
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A0F5GET7
• Locus tag: C6992_RS17010
• Protein ID: WP_000227783.1
• Coordinates: 3117645..3118187 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C6992_RS17005
• Protein ID: WP_001297137.1
• Coordinates: 3117350..3117661 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS16980	3112370..3113317	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C6992_RS16985	3113339..3115330	+	1992	WP_032314628.1	cytochrome o ubiquinol oxidase subunit I	-
C6992_RS16990	3115320..3115934	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C6992_RS16995	3115934..3116263	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C6992_RS17000	3116275..3117165	+	891	WP_032314627.1	heme o synthase	-
C6992_RS17005	3117350..3117661	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C6992_RS17010	3117645..3118187	+	543	WP_000227783.1	GNAT family N-acetyltransferase	Toxin
C6992_RS17015	3118243..3119178	-	936	WP_001297127.1	sel1 repeat family protein	-
C6992_RS17020	3119586..3120950	+	1365	WP_032314626.1	MFS transporter	-
C6992_RS17025	3121078..3121569	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C6992_RS17030	3121737..3122648	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.96 Da
• Isoelectric Point: 8.0330

>T99353 WP_000227783.1 NZ_CP027548:3117645-3118187 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99353 NZ_CP027548:3117645-3118187 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99353 WP_001297137.1 NZ_CP027548:3117350-3117661 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99353 NZ_CP027548:3117350-3117661 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0F5GET7

Antitoxin

• AlphaFold DB: A0A0E0Y6B3