Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1836208..1836846 Replicon chromosome
Accession NZ_CP027548
Organism Escherichia coli strain 2014C-3061

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6992_RS09830 Protein ID WP_000813794.1
Coordinates 1836670..1836846 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6992_RS09825 Protein ID WP_001270286.1
Coordinates 1836208..1836624 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6992_RS09805 1831360..1832301 - 942 WP_032314702.1 ABC transporter permease -
C6992_RS09810 1832302..1833315 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6992_RS09815 1833333..1834478 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6992_RS09820 1834723..1836129 - 1407 WP_000760615.1 PLP-dependent aminotransferase family protein -
C6992_RS09825 1836208..1836624 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6992_RS09830 1836670..1836846 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6992_RS09835 1837068..1837298 + 231 WP_000494244.1 YncJ family protein -
C6992_RS09840 1837390..1839351 - 1962 WP_032314701.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6992_RS09845 1839424..1839960 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6992_RS09850 1840013..1841227 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1841267..1842415 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99350 WP_000813794.1 NZ_CP027548:c1836846-1836670 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99350 NZ_CP027548:c1836846-1836670 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99350 WP_001270286.1 NZ_CP027548:c1836624-1836208 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99350 NZ_CP027548:c1836624-1836208 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References