Detailed information of TA system TA099335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 580569..581194
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6992_RS03025
• Protein ID: WP_000911330.1
• Coordinates: 580796..581194 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6992_RS03020
• Protein ID: WP_000450524.1
• Coordinates: 580569..580796 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS02995	576373..576843	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6992_RS03000	576843..577415	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6992_RS03005	577561..578439	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6992_RS03010	578456..579490	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6992_RS03015	579703..580416	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6992_RS03020	580569..580796	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6992_RS03025	580796..581194	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6992_RS03030	581341..582204	+	864	WP_001267495.1	neutral zinc metallopeptidase	-
C6992_RS03035	582219..584234	+	2016	WP_032314290.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6992_RS03040	584308..585006	+	699	WP_106904288.1	esterase	-
C6992_RS03045	585116..585316	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99335 WP_000911330.1 NZ_CP027548:580796-581194 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99335 NZ_CP027548:580796-581194 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99335 WP_000450524.1 NZ_CP027548:580569-580796 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99335 NZ_CP027548:580569-580796 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33