Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 71199..71853 Replicon chromosome
Accession NZ_CP027548
Organism Escherichia coli strain 2014C-3061

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6992_RS00410 Protein ID WP_000244777.1
Coordinates 71446..71853 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6992_RS00405 Protein ID WP_000354046.1
Coordinates 71199..71465 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6992_RS00380 66368..67111 + 744 WP_000951964.1 SDR family oxidoreductase -
C6992_RS00385 67168..68601 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
C6992_RS00390 68646..68957 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6992_RS00395 69121..69780 + 660 WP_032314344.1 hemolysin III family protein -
C6992_RS00400 69976..70956 - 981 WP_106904278.1 tRNA-modifying protein YgfZ -
C6992_RS00405 71199..71465 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6992_RS00410 71446..71853 + 408 WP_000244777.1 toxin CptA Toxin
C6992_RS00415 71893..72414 - 522 WP_001055874.1 flavodoxin FldB -
C6992_RS00420 72526..73422 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6992_RS00425 73447..74157 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6992_RS00430 74163..75896 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99331 WP_000244777.1 NZ_CP027548:71446-71853 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99331 NZ_CP027548:71446-71853 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99331 WP_000354046.1 NZ_CP027548:71199-71465 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99331 NZ_CP027548:71199-71465 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References