Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71960..72603 Replicon plasmid unnamed
Accession NZ_CP027547
Organism Escherichia coli strain 2013C-4187

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6993_RS30055 Protein ID WP_001044768.1
Coordinates 71960..72376 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6993_RS30060 Protein ID WP_001261287.1
Coordinates 72373..72603 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6993_RS30030 67122..67796 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
C6993_RS30035 67850..68236 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
C6993_RS30040 68364..69224 + 861 WP_000704534.1 alpha/beta hydrolase -
C6993_RS30595 69713..69988 + 276 WP_157776129.1 hypothetical protein -
C6993_RS30050 71253..71798 + 546 WP_001165114.1 hypothetical protein -
C6993_RS30055 71960..72376 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6993_RS30060 72373..72603 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6993_RS30075 73165..73578 + 414 WP_000465041.1 hypothetical protein -
C6993_RS30080 73580..74362 + 783 WP_001164205.1 site-specific integrase -
C6993_RS30085 74535..74882 + 348 WP_106889347.1 colicin M immunity protein -
C6993_RS30090 74934..76090 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
C6993_RS30095 76163..76354 - 192 Protein_79 lipid II-degrading bacteriocin -
C6993_RS30100 76358..76501 - 144 Protein_80 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / katA / toxB / toxB / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC 1..95367 95367
- inside IScluster/Tn - - 66778..76090 9312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99330 WP_001044768.1 NZ_CP027547:c72376-71960 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99330 NZ_CP027547:c72376-71960 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99330 WP_001261287.1 NZ_CP027547:c72603-72373 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99330 NZ_CP027547:c72603-72373 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References