Detailed information of TA system TA099316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2387711..2388336
• Replicon: chromosome
• Accession: NZ_CP027546
• Organism: Escherichia coli strain 2013C-4187

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6993_RS13390
• Protein ID: WP_000911330.1
• Coordinates: 2387711..2388109 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6993_RS13395
• Protein ID: WP_000450524.1
• Coordinates: 2388109..2388336 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6993_RS13370	2383589..2383789	+	201	WP_000383836.1	YpfN family protein	-
C6993_RS13375	2383899..2384597	-	699	WP_000679812.1	esterase	-
C6993_RS13380	2384671..2386686	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6993_RS13385	2386701..2387564	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6993_RS13390	2387711..2388109	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6993_RS13395	2388109..2388336	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6993_RS13400	2388490..2389203	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6993_RS13405	2389416..2390450	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6993_RS13410	2390467..2391345	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6993_RS13415	2391491..2392063	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6993_RS13420	2392063..2392533	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99316 WP_000911330.1 NZ_CP027546:c2388109-2387711 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99316 NZ_CP027546:c2388109-2387711 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99316 WP_000450524.1 NZ_CP027546:c2388336-2388109 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99316 NZ_CP027546:c2388336-2388109 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33