Detailed information of TA system TA099306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1564399..1564870
• Replicon: chromosome
• Accession: NZ_CP027546
• Organism: Escherichia coli strain 2013C-4187

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C6993_RS09090
• Protein ID: WP_001303511.1
• Coordinates: 1564592..1564870 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C6993_RS09085
• Protein ID: WP_001302048.1
• Coordinates: 1564399..1564590 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6993_RS09035	1559477..1559695	-	219	WP_000683609.1	DUF4014 family protein	-
C6993_RS09040	1559823..1560134	-	312	WP_001289673.1	hypothetical protein	-
C6993_RS09045	1560127..1560354	-	228	WP_000699809.1	hypothetical protein	-
C6993_RS09050	1560351..1560632	-	282	WP_000603384.1	DNA-binding protein	-
C6993_RS09055	1560665..1561426	-	762	WP_000450872.1	DUF1627 domain-containing protein	-
C6993_RS09060	1561452..1562193	-	742	Protein_1653	ATP-binding protein	-
C6993_RS09065	1562200..1563162	-	963	WP_001444941.1	helix-turn-helix domain-containing protein	-
C6993_RS09070	1563185..1563610	-	426	WP_000693943.1	Rha family transcriptional regulator	-
C6993_RS09075	1563607..1563909	-	303	WP_000172738.1	transcriptional regulator	-
C6993_RS09080	1564007..1564378	+	372	WP_001169687.1	hypothetical protein	-
C6993_RS09085	1564399..1564590	+	192	WP_001302048.1	hypothetical protein	Antitoxin
C6993_RS09090	1564592..1564870	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6993_RS09095	1565157..1565309	+	153	WP_001427316.1	DUF1391 family protein	-
C6993_RS09100	1565730..1565951	+	222	WP_000560218.1	killing protein KilR	-
C6993_RS09105	1565945..1566121	+	177	WP_000245534.1	hypothetical protein	-
C6993_RS09110	1566195..1566470	+	276	WP_001307773.1	hypothetical protein	-
C6993_RS09115	1566569..1569220	+	2652	WP_000105101.1	exodeoxyribonuclease VIII	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	1522358..1579032	56674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T99306 WP_001303511.1 NZ_CP027546:1564592-1564870 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T99306 NZ_CP027546:1564592-1564870 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT99306 WP_001302048.1 NZ_CP027546:1564399-1564590 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT99306 NZ_CP027546:1564399-1564590 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0