Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 837921..838559 Replicon chromosome
Accession NZ_CP027546
Organism Escherichia coli strain 2013C-4187

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6993_RS04955 Protein ID WP_000813794.1
Coordinates 837921..838097 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6993_RS04960 Protein ID WP_001270286.1
Coordinates 838143..838559 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6993_RS04935 833540..834754 - 1215 WP_106913817.1 BenE family transporter YdcO -
C6993_RS04940 834807..835343 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6993_RS04945 835416..837377 + 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6993_RS04950 837469..837699 - 231 WP_000494244.1 YncJ family protein -
C6993_RS04955 837921..838097 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6993_RS04960 838143..838559 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6993_RS04965 838638..840043 + 1406 Protein_900 PLP-dependent aminotransferase family protein -
C6993_RS04970 840288..841433 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6993_RS04975 841451..842464 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6993_RS04980 842465..843406 + 942 WP_001251332.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 832352..833500 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99304 WP_000813794.1 NZ_CP027546:837921-838097 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99304 NZ_CP027546:837921-838097 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99304 WP_001270286.1 NZ_CP027546:838143-838559 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99304 NZ_CP027546:838143-838559 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References