Detailed information of TA system TA099300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 5730..6265
• Replicon: plasmid unnamed
• Accession: NZ_CP027545
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MJ68_RS29665
• Protein ID: WP_001270421.1
• Coordinates: 5730..6017 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: MJ68_RS29670
• Protein ID: WP_001132893.1
• Coordinates: 6014..6265 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS29635	849..1589	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
MJ68_RS29640	1873..2850	-	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
MJ68_RS29650	3496..3784	-	289	Protein_5	integrase core domain-containing protein	-
MJ68_RS29655	3969..5155	-	1187	Protein_6	IS91 family transposase	-
MJ68_RS29660	5155..5520	-	366	WP_000091308.1	hypothetical protein	-
MJ68_RS30860	5606..5728	-	123	Protein_8	IS3 family transposase	-
MJ68_RS29665	5730..6017	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ68_RS29670	6014..6265	-	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJ68_RS29675	6654..7859	+	1206	WP_000817642.1	AAA family ATPase	-
MJ68_RS29680	7856..8818	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
MJ68_RS29685	8958..9326	-	369	Protein_13	DUF4113 domain-containing protein	-
MJ68_RS29690	9514..10700	-	1187	Protein_14	IS91 family transposase	-
MJ68_RS29695	10700..11065	-	366	WP_000091308.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / stcE / stcE / stcE / exeE / exeG / espP / nleA/espI	1..101089	101089
	-	inside	IScluster/Tn	-	-	3969..10236	6267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T99300 WP_001270421.1 NZ_CP027545:c6017-5730 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T99300 NZ_CP027545:c6017-5730 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT99300 WP_001132893.1 NZ_CP027545:c6265-6014 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT99300 NZ_CP027545:c6265-6014 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724