Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4360579..4361197 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MJ68_RS23570 Protein ID WP_001291435.1
Coordinates 4360579..4360797 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MJ68_RS23575 Protein ID WP_000344800.1
Coordinates 4360823..4361197 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS23535 4355869..4356441 + 573 WP_000779826.1 lipoprotein -
MJ68_RS23540 4356472..4356783 - 312 WP_000409911.1 MGMT family protein -
MJ68_RS23550 4357162..4357515 + 354 WP_000878140.1 DUF1428 domain-containing protein -
MJ68_RS23555 4357557..4359107 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MJ68_RS23560 4359271..4359741 - 471 WP_000136192.1 YlaC family protein -
MJ68_RS23565 4359857..4360408 - 552 WP_000102542.1 maltose O-acetyltransferase -
MJ68_RS23570 4360579..4360797 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
MJ68_RS23575 4360823..4361197 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MJ68_RS23580 4361743..4364892 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
MJ68_RS23585 4364915..4366108 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99290 WP_001291435.1 NZ_CP027544:c4360797-4360579 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99290 NZ_CP027544:c4360797-4360579 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99290 WP_000344800.1 NZ_CP027544:c4361197-4360823 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99290 NZ_CP027544:c4361197-4360823 [Escherichia coli]
ATGGATGAATACTCACCTAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References