Detailed information of TA system TA099289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4326274..4327111
• Replicon: chromosome
• Accession: NZ_CP027544
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A0F5GET7
• Locus tag: MJ68_RS23390
• Protein ID: WP_000227783.1
• Coordinates: 4326274..4326816 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MJ68_RS23395
• Protein ID: WP_001297137.1
• Coordinates: 4326800..4327111 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS23370	4321813..4322724	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
MJ68_RS23375	4322892..4323383	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
MJ68_RS23380	4323511..4324875	-	1365	WP_001000942.1	MFS transporter	-
MJ68_RS23385	4325283..4326218	+	936	WP_001297127.1	sel1 repeat family protein	-
MJ68_RS23390	4326274..4326816	-	543	WP_000227783.1	GNAT family N-acetyltransferase	Toxin
MJ68_RS23395	4326800..4327111	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MJ68_RS23400	4327296..4328186	-	891	WP_000971327.1	heme o synthase	-
MJ68_RS23405	4328198..4328527	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MJ68_RS23410	4328527..4329141	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MJ68_RS23415	4329131..4331122	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MJ68_RS23420	4331144..4332100	-	957	WP_001239442.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.96 Da
• Isoelectric Point: 8.0330

>T99289 WP_000227783.1 NZ_CP027544:c4326816-4326274 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99289 NZ_CP027544:c4326816-4326274 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCAGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99289 WP_001297137.1 NZ_CP027544:c4327111-4326800 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99289 NZ_CP027544:c4327111-4326800 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0F5GET7

Antitoxin

• AlphaFold DB: A0A0E0Y6B3