Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3630178..3630773 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag MJ68_RS19855 Protein ID WP_000239579.1
Coordinates 3630423..3630773 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A240D6C8
Locus tag MJ68_RS19850 Protein ID WP_001223212.1
Coordinates 3630178..3630429 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS19840 3625844..3629622 + 3779 Protein_3585 autotransporter assembly complex protein TamB -
MJ68_RS19845 3629625..3629966 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
MJ68_RS19850 3630178..3630429 + 252 WP_001223212.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
MJ68_RS19855 3630423..3630773 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
MJ68_RS19860 3630853..3631383 - 531 WP_000055072.1 inorganic diphosphatase -
MJ68_RS19865 3631693..3632649 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
MJ68_RS19870 3632789..3634291 + 1503 WP_000210553.1 sugar ABC transporter ATP-binding protein -
MJ68_RS19875 3634305..3635327 + 1023 WP_001353963.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T99287 WP_000239579.1 NZ_CP027544:3630423-3630773 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T99287 NZ_CP027544:3630423-3630773 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTTGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.6509

>AT99287 WP_001223212.1 NZ_CP027544:3630178-3630429 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT99287 NZ_CP027544:3630178-3630429 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A240D6C8

References