Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2675300..2675931 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag MJ68_RS14930 Protein ID WP_001259385.1
Coordinates 2675656..2675931 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MJ68_RS14925 Protein ID WP_000593555.1
Coordinates 2675300..2675659 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS14900 2671432..2672376 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
MJ68_RS14905 2672373..2673206 + 834 WP_001008966.1 nickel ABC transporter permease subunit NikC -
MJ68_RS14910 2673206..2673970 + 765 WP_001136215.1 nickel import ATP-binding protein NikD -
MJ68_RS14915 2673967..2674773 + 807 WP_000173711.1 nickel import ATP-binding protein NikE -
MJ68_RS14920 2674779..2675180 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MJ68_RS14925 2675300..2675659 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MJ68_RS14930 2675656..2675931 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
MJ68_RS14935 2676043..2677167 - 1125 WP_001216257.1 ABC transporter permease -
MJ68_RS14940 2677167..2679902 - 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T99282 WP_001259385.1 NZ_CP027544:c2675931-2675656 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99282 NZ_CP027544:c2675931-2675656 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99282 WP_000593555.1 NZ_CP027544:c2675659-2675300 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99282 NZ_CP027544:c2675659-2675300 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References