Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2208048..2208885 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7U9LL67
Locus tag MJ68_RS12330 Protein ID WP_000854687.1
Coordinates 2208048..2208428 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LJJ3
Locus tag MJ68_RS12335 Protein ID WP_001443392.1
Coordinates 2208517..2208885 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS12300 2203691..2205532 + 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -
MJ68_RS12305 2205610..2206116 - 507 WP_000228940.1 G/U mismatch-specific DNA glycosylase -
MJ68_RS12315 2206416..2207261 - 846 WP_001274557.1 DUF4942 domain-containing protein -
MJ68_RS12320 2207346..2207543 - 198 WP_000772026.1 DUF957 domain-containing protein -
MJ68_RS12325 2207563..2208051 - 489 WP_001054228.1 hypothetical protein -
MJ68_RS12330 2208048..2208428 - 381 WP_000854687.1 TA system toxin CbtA family protein Toxin
MJ68_RS12335 2208517..2208885 - 369 WP_001443392.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MJ68_RS12340 2208960..2209181 - 222 WP_001220313.1 DUF987 domain-containing protein -
MJ68_RS12345 2209250..2209726 - 477 WP_001186714.1 RadC family protein -
MJ68_RS12350 2209742..2210215 - 474 WP_001397416.1 antirestriction protein -
MJ68_RS30965 2210401..2210553 - 153 WP_176079112.1 hypothetical protein -
MJ68_RS12360 2210553..2210992 - 440 Protein_2256 DUF932 domain-containing protein -
MJ68_RS12365 2211110..2212887 - 1778 Protein_2257 reverse transcriptase -
MJ68_RS12370 2213397..2213780 - 384 Protein_2258 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14199.13 Da        Isoelectric Point: 6.8615

>T99279 WP_000854687.1 NZ_CP027544:c2208428-2208048 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Download         Length: 381 bp

>T99279 NZ_CP027544:c2208428-2208048 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13781.54 Da        Isoelectric Point: 6.2049

>AT99279 WP_001443392.1 NZ_CP027544:c2208885-2208517 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Download         Length: 369 bp

>AT99279 NZ_CP027544:c2208885-2208517 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LL67

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LJJ3

References