Detailed information of TA system TA099272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5829255..5829896
• Replicon: chromosome
• Accession: NZ_CP027541
• Organism: Mycolicibacterium smegmatis MKD8

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: D806_RS28235
• Protein ID: WP_011730672.1
• Coordinates: 5829255..5829698 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A2U9PXD6
• Locus tag: D806_RS28240
• Protein ID: WP_003897042.1
• Coordinates: 5829708..5829896 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D806_RS28215	5824741..5825478	+	738	WP_003897037.1	GntR family transcriptional regulator	-
D806_RS28220	5825480..5827009	+	1530	WP_003897038.1	cyclohexanecarboxylate-CoA ligase	-
D806_RS28225	5827010..5827783	+	774	WP_003897039.1	SDR family oxidoreductase	-
D806_RS28230	5827780..5829252	+	1473	WP_003897040.1	long-chain fatty acid--CoA ligase	-
D806_RS28235	5829255..5829698	-	444	WP_011730672.1	SRPBCC family protein	Toxin
D806_RS28240	5829708..5829896	-	189	WP_003897042.1	antitoxin	Antitoxin
D806_RS28245	5829922..5832297	-	2376	WP_003897043.1	cation-translocating P-type ATPase	-
D806_RS28250	5832311..5833918	-	1608	WP_003897044.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T99272 WP_011730672.1 NZ_CP027541:c5829698-5829255 [Mycolicibacterium smegmatis MKD8]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T99272 NZ_CP027541:c5829698-5829255 [Mycolicibacterium smegmatis MKD8]
ATGGCCAAACTTTCCGTCTCCGTCGAAGTTCCTTTGCCGCCGGAGCAGGCGTGGGAGTATGCCTCCGACCTGTCCCGGTA
CCACGAGTGGCTCAGCATTCACCGGGCCTGGCGTTCGAAGTTGCCCGAGACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAGGTGGACGCTGGTCCACTACAAGCCCCCGCAGGCGCTCACACTCAAC
GGTGACGGCCGCGGCGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCCACCGACAACGGCGCGACGGTGGGCTTCGA
CCTGCACCTCGGCGGCCCGGCCCTGTTCGGGCCCATCGGGATGGTGGTGGCCGCGGCGCTCAAGAGCGACATTCAGGAGT
CGCTCAACCGCTTCAAGCAGCTCTACGCCCCGTCCGCGACCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6886.64 Da
• Isoelectric Point: 4.6906

>AT99272 WP_003897042.1 NZ_CP027541:c5829896-5829708 [Mycolicibacterium smegmatis MKD8]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIRKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT99272 NZ_CP027541:c5829896-5829708 [Mycolicibacterium smegmatis MKD8]
ATGGGATTCCTGGACAAGGCCAAAGACCTCTTGTCGCAGAACGCCGACAAGGTGGACACCGTCATTGACAAGGCCGGCGA
CATGATCGACGAGAAGACGCAGGGCAAGTACGCCCAGCACGTCGACAAGGCGCAGGACGCCGCGAAGAACGCCATCCGCA
AAGACGACCCTCAAGAACCACAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PXD6