Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4565305..4565846 Replicon chromosome
Accession NZ_CP027541
Organism Mycolicibacterium smegmatis MKD8

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag D806_RS21990 Protein ID WP_003895797.1
Coordinates 4565517..4565846 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag D806_RS21985 Protein ID WP_011729843.1
Coordinates 4565305..4565514 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D806_RS21965 4560775..4561152 + 378 WP_003895792.1 hypothetical protein -
D806_RS21970 4561160..4562242 - 1083 WP_198670891.1 zinc-binding dehydrogenase -
D806_RS21975 4562239..4562940 - 702 WP_003895794.1 phosphonatase-like hydrolase -
D806_RS21980 4563114..4564934 + 1821 WP_080628131.1 chloride channel protein -
D806_RS21985 4565305..4565514 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
D806_RS21990 4565517..4565846 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D806_RS21995 4565889..4566452 - 564 WP_014878038.1 TetR/AcrR family transcriptional regulator -
D806_RS22000 4566558..4567493 + 936 WP_003895799.1 alpha/beta fold hydrolase -
D806_RS22005 4567490..4569022 + 1533 WP_036453403.1 NAD(P)/FAD-dependent oxidoreductase -
D806_RS22020 4569516..4569896 + 381 WP_029104585.1 phage holin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T99271 WP_003895797.1 NZ_CP027541:4565517-4565846 [Mycolicibacterium smegmatis MKD8]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T99271 NZ_CP027541:4565517-4565846 [Mycolicibacterium smegmatis MKD8]
GTGCGGCGCGGCGATATCTACACCGCGGCGGCGCGAGGTGCGTACACCGGCAAGCCTCGGCCGGTCCTCATCATCCAGGA
CGACCGATTCGATGCCACCGCGTCGGTCACCGTCGTGCCGTTTACGACAAGCGACACCGACGCACCGCTTCTGCGAATTC
GAATCGAGCCAACGCCAGCCACCGGACTCTCCACGGTCAGCTCGTTGATGATCGACAAGGTGACCACAGTTCCCCGCTCC
AGCTTGACGCACCGTGTCGGCCGACTGGCGGAGACAGACATGGTCAGGACCGACCGCGCACTCCTGGTTTTTCTGGGAAT
CGCCGGGTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT99271 WP_011729843.1 NZ_CP027541:4565305-4565514 [Mycolicibacterium smegmatis MKD8]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT99271 NZ_CP027541:4565305-4565514 [Mycolicibacterium smegmatis MKD8]
ATGACTCCCGCGCGTGACCGAGTACGCCGACATCGCGAGCGGCTGCGGCGACAAGGTCTGCGGCCGGTGCAGATCTGGGT
CCCCGACGTGAATGCACCCGAATTTCGCCGGGAGGCGCACCGGCAGTCTGAGTTGGTCGCCGCCGGCGAGCATGAGGCCG
AAGATCAAGCTTTCGTCGACGCGATCTCCGTCGACTGGGACGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References