Detailed information of TA system TA099261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4478483..4479008
• Replicon: chromosome
• Accession: NZ_CP027539
• Organism: Serratia marcescens strain AR_0099

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AM478_RS21230
• Protein ID: WP_033646666.1
• Coordinates: 4478724..4479008 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8B4GIN1
• Locus tag: AM478_RS21225
• Protein ID: WP_004928423.1
• Coordinates: 4478483..4478734 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM478_RS21195	4473754..4474296	+	543	WP_033637821.1	lipoprotein	-
AM478_RS21200	4474561..4475289	+	729	WP_038884338.1	arginine ABC transporter ATP-binding protein ArtP	-
AM478_RS21205	4475322..4476053	+	732	WP_033646663.1	arginine ABC transporter substrate-binding protein	-
AM478_RS21210	4476063..4476779	+	717	WP_033646665.1	arginine ABC transporter permease ArtQ	-
AM478_RS21215	4476779..4477447	+	669	WP_033637817.1	arginine ABC transporter permease ArtM	-
AM478_RS21220	4477647..4478381	+	735	WP_033637816.1	arginine ABC transporter substrate-binding protein	-
AM478_RS21225	4478483..4478734	+	252	WP_004928423.1	prevent-host-death protein	Antitoxin
AM478_RS21230	4478724..4479008	+	285	WP_033646666.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM478_RS21235	4479005..4480132	-	1128	WP_033646667.1	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC	-
AM478_RS21240	4480202..4480681	-	480	WP_048321404.1	YbjO family protein	-
AM478_RS21245	4480786..4481631	-	846	WP_016928360.1	putrescine ABC transporter permease PotI	-
AM478_RS21250	4481628..4482590	-	963	WP_033646669.1	putrescine ABC transporter permease PotH	-
AM478_RS21255	4482615..4483748	-	1134	WP_033637812.1	putrescine ABC transporter ATP-binding subunit PotG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10797.98 Da
• Isoelectric Point: 10.7537

>T99261 WP_033646666.1 NZ_CP027539:4478724-4479008 [Serratia marcescens]
MTYKLEFEEHALKEFKKLSPVIREQFKKKLVSVLVNPHVPANKLAGLPDCYKIKLRASGFRLVYRVMETKIVVLVLSVGK
REHSAAYTAAKKRL

Nucleotide

• Length: 285 bp

>T99261 NZ_CP027539:4478724-4479008 [Serratia marcescens]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGTATCGGTATTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCCGATTGCTACAAAATCA
AACTCAGAGCATCAGGCTTTCGTTTGGTGTATCGCGTCATGGAAACGAAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACATTCAGCGGCTTATACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9108.39 Da
• Isoelectric Point: 4.5203

>AT99261 WP_004928423.1 NZ_CP027539:4478483-4478734 [Serratia marcescens]
MTVQPILADSAVSISDFKKSPNAALKEAQGQPVAVLTNGKISGYYVSPETWEAIAEYLEDIELAEIVRSRMNGKRIKVNL
DDL

Nucleotide

• Length: 252 bp

>AT99261 NZ_CP027539:4478483-4478734 [Serratia marcescens]
ATGACGGTTCAACCCATTCTTGCCGATTCCGCAGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAGCCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCACCGGAAACCTGGGAGGCTA
TCGCGGAGTACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GIN1