Detailed information of TA system TA099249

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1994152..1994821
• Replicon: chromosome
• Accession: NZ_CP027539
• Organism: Serratia marcescens strain AR_0099

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A2V4FX75
• Locus tag: AM478_RS09395
• Protein ID: WP_033649453.1
• Coordinates: 1994399..1994821 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A8G2G4M4
• Locus tag: AM478_RS09390
• Protein ID: WP_004931679.1
• Coordinates: 1994152..1994418 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM478_RS09370	1990686..1991294	-	609	WP_025304127.1	HD domain-containing protein	-
AM478_RS09375	1991478..1992152	+	675	WP_033649450.1	hemolysin III family protein	-
AM478_RS09380	1992303..1992800	+	498	WP_033649451.1	DUF2165 domain-containing protein	-
AM478_RS09385	1992839..1993831	-	993	WP_033649452.1	tRNA-modifying protein YgfZ	-
AM478_RS09390	1994152..1994418	+	267	WP_004931679.1	FAD assembly factor SdhE	Antitoxin
AM478_RS09395	1994399..1994821	+	423	WP_033649453.1	protein YgfX	Toxin
AM478_RS09400	1994821..1995516	+	696	WP_033649454.1	two-component system response regulator CreB	-
AM478_RS09405	1995513..1996931	+	1419	WP_033649455.1	two-component system sensor histidine kinase CreC	-
AM478_RS09410	1997012..1998376	+	1365	WP_033649456.1	cell envelope integrity protein CreD	-
AM478_RS09415	1998414..1998932	-	519	WP_033635825.1	flavodoxin FldB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16471.59 Da
• Isoelectric Point: 10.8114

>T99249 WP_033649453.1 NZ_CP027539:1994399-1994821 [Serratia marcescens]
VAQWRCDIRISWRTQLFSLLTHGVLILLILISPWPEGFGPLWLVLLTLVVFQCIRSQKRIAAAQGELRLLADRRFSWHGR
EWRLAKKPWMPGYGMLLTLLPMEGKKRRRLWLASDCMSKEEWRHLRQLLLYPPAGDGEDP

Nucleotide

• Length: 423 bp

>T99249 NZ_CP027539:1994399-1994821 [Serratia marcescens]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTGACCCACGGCGTGTTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGTTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGCGCAGGGCGAGCTGCGGCTGCTGGCGGACCGGCGCTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGCTGACGCTGCTGCCGATGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
CGGGTGACGGCGAGGATCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10387.89 Da
• Isoelectric Point: 4.7921

>AT99249 WP_004931679.1 NZ_CP027539:1994152-1994418 [Serratia marcescens]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLNDADKALFIRLLECDDPDLFNWLMNHGAPQDGELQRMVTLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT99249 NZ_CP027539:1994152-1994418 [Serratia marcescens]
ATGGATATTAACAACAAAGCACGAATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAGTACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A2V4FX75