Detailed information of TA system TA099248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1707099..1707694
• Replicon: chromosome
• Accession: NZ_CP027539
• Organism: Serratia marcescens strain AR_0099

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: AM478_RS08040
• Protein ID: WP_071605322.1
• Coordinates: 1707099..1707353 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM478_RS08045
• Protein ID: WP_033633573.1
• Coordinates: 1707353..1707694 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM478_RS08015	1702148..1704361	+	2214	WP_033649032.1	replication endonuclease	-
AM478_RS08020	1704402..1704632	+	231	WP_049299940.1	hypothetical protein	-
AM478_RS08025	1704680..1704877	+	198	WP_139818298.1	hypothetical protein	-
AM478_RS08030	1704974..1705873	-	900	WP_033649106.1	LysR family transcriptional regulator	-
AM478_RS08035	1706002..1706574	+	573	WP_019843167.1	flavin reductase	-
AM478_RS08040	1707099..1707353	+	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM478_RS08045	1707353..1707694	+	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM478_RS08050	1707739..1708773	-	1035	WP_033649034.1	phage portal protein	-
AM478_RS08055	1708773..1710545	-	1773	WP_033649036.1	terminase ATPase subunit family protein	-
AM478_RS08060	1710688..1711503	+	816	WP_033649037.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	-	1696292..1739918	43626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T99248 WP_071605322.1 NZ_CP027539:1707099-1707353 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T99248 NZ_CP027539:1707099-1707353 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT99248 WP_033633573.1 NZ_CP027539:1707353-1707694 [Serratia marcescens]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT99248 NZ_CP027539:1707353-1707694 [Serratia marcescens]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCTACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1