Detailed information of TA system TA099238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 2895404..2895909
• Replicon: chromosome
• Accession: NZ_CP027538
• Organism: Pseudomonas aeruginosa strain AR_0095

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: AM474_RS14165
• Protein ID: WP_003083773.1
• Coordinates: 2895628..2895909 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: AM474_RS14160
• Protein ID: WP_034028307.1
• Coordinates: 2895404..2895631 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM474_RS14135	2890422..2891915	+	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
AM474_RS14140	2892084..2893511	+	1428	WP_003083784.1	GABA permease	-
AM474_RS14145	2893593..2893934	-	342	WP_003101229.1	alkylphosphonate utilization protein	-
AM474_RS14150	2894007..2894507	-	501	WP_003162763.1	LEA type 2 family protein	-
AM474_RS14155	2894608..2895228	+	621	WP_003101226.1	hypothetical protein	-
AM474_RS14160	2895404..2895631	+	228	WP_034028307.1	CopG family ribbon-helix-helix protein	Antitoxin
AM474_RS14165	2895628..2895909	+	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
AM474_RS14170	2896209..2897117	+	909	WP_034028305.1	LysR family transcriptional regulator	-
AM474_RS14175	2897149..2897535	-	387	WP_003083767.1	aegerolysin family protein	-
AM474_RS14180	2897739..2898473	-	735	WP_003110658.1	GntR family transcriptional regulator	-
AM474_RS14185	2898574..2899260	-	687	WP_003083762.1	FadR family transcriptional regulator	-
AM474_RS14190	2899309..2900658	-	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T99238 WP_003083773.1 NZ_CP027538:2895628-2895909 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T99238 NZ_CP027538:2895628-2895909 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATATACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8584.64 Da
• Isoelectric Point: 5.5740

>AT99238 WP_034028307.1 NZ_CP027538:2895404-2895631 [Pseudomonas aeruginosa]
MSTVVSFRADDVLVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT99238 NZ_CP027538:2895404-2895631 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGTCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW