Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 40040..40641 Replicon plasmid unnamed2
Accession NZ_CP027537
Organism Escherichia coli strain AR_0081

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag AM460_RS27765 Protein ID WP_001216034.1
Coordinates 40261..40641 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag AM460_RS27760 Protein ID WP_001190712.1
Coordinates 40040..40261 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM460_RS27750 37033..38304 - 1272 WP_023142242.1 restriction endonuclease subunit S -
AM460_RS27755 38301..39857 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
AM460_RS27760 40040..40261 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AM460_RS27765 40261..40641 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
AM460_RS27770 40646..40825 + 180 WP_001513661.1 hypothetical protein -
AM460_RS27775 40853..41212 + 360 WP_001513660.1 hypothetical protein -
AM460_RS27780 41136..41549 + 414 Protein_51 DDE-type integrase/transposase/recombinase -
AM460_RS27785 41499..41816 - 318 WP_001513659.1 hypothetical protein -
AM460_RS27790 42044..43024 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
AM460_RS27795 43268..44467 + 1200 Protein_54 S-methylmethionine permease -
AM460_RS27800 44532..45236 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
AM460_RS28405 45242..45433 + 192 WP_158701885.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..100279 100279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T99231 WP_001216034.1 NZ_CP027537:40261-40641 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T99231 NZ_CP027537:40261-40641 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT99231 WP_001190712.1 NZ_CP027537:40040-40261 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT99231 NZ_CP027537:40040-40261 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References