Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 5029..5576 Replicon plasmid unnamed1
Accession NZ_CP027535
Organism Escherichia coli strain AR_0081

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag AM460_RS26375 Protein ID WP_000969996.1
Coordinates 5029..5310 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag AM460_RS26380 Protein ID WP_000079928.1
Coordinates 5307..5576 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM460_RS26335 96..692 + 597 WP_046340578.1 proQ/FINO family protein -
AM460_RS26340 748..1251 + 504 WP_046340576.1 hypothetical protein -
AM460_RS26350 1419..1691 + 273 WP_000019163.1 hypothetical protein -
AM460_RS26355 1672..2880 + 1209 WP_000198533.1 IS91 family transposase -
AM460_RS26365 3132..3404 + 273 WP_000019163.1 hypothetical protein -
AM460_RS26370 3385..4593 + 1209 WP_000198533.1 IS91 family transposase -
AM460_RS26375 5029..5310 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM460_RS26380 5307..5576 - 270 WP_000079928.1 hypothetical protein Antitoxin
AM460_RS26390 6487..7518 - 1032 WP_000907854.1 hypothetical protein -
AM460_RS26405 8491..8754 + 264 WP_001324596.1 ash family protein -
AM460_RS26410 8672..8959 + 288 WP_000074849.1 conjugal transfer protein TraA -
AM460_RS26420 9401..9934 + 534 WP_001303319.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 - 1..101207 101207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T99213 WP_000969996.1 NZ_CP027535:c5310-5029 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T99213 NZ_CP027535:c5310-5029 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT99213 WP_000079928.1 NZ_CP027535:c5576-5307 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT99213 NZ_CP027535:c5576-5307 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References