Detailed information of TA system TA099171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1295029..1295624
• Replicon: chromosome
• Accession: NZ_CP027533
• Organism: Serratia marcescens strain AR_0091

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: AM470_RS06340
• Protein ID: WP_071605322.1
• Coordinates: 1295029..1295283 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM470_RS06345
• Protein ID: WP_063988754.1
• Coordinates: 1295283..1295624 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM470_RS06295	1290087..1290275	-	189	WP_037432527.1	hypothetical protein	-
AM470_RS06300	1290422..1290601	+	180	WP_072269687.1	DUF2724 domain-containing protein	-
AM470_RS06305	1290613..1290915	+	303	WP_060418375.1	hypothetical protein	-
AM470_RS06310	1290980..1291273	+	294	WP_060418376.1	DUF2732 family protein	-
AM470_RS06315	1291273..1291497	+	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
AM470_RS06320	1291623..1291937	+	315	WP_060418377.1	DUF3850 domain-containing protein	-
AM470_RS06325	1291934..1294150	+	2217	WP_106046294.1	replication endonuclease	-
AM470_RS06330	1294189..1294413	+	225	WP_033644912.1	hypothetical protein	-
AM470_RS06335	1294461..1294658	+	198	WP_126186641.1	hypothetical protein	-
AM470_RS06340	1295029..1295283	+	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM470_RS06345	1295283..1295624	+	342	WP_063988754.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM470_RS06350	1295668..1296885	+	1218	WP_086556860.1	FRG domain-containing protein	-
AM470_RS06355	1296920..1297954	-	1035	WP_086556861.1	phage portal protein	-
AM470_RS06360	1297954..1299726	-	1773	WP_086556862.1	terminase ATPase subunit family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1284960..1318987	34027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T99171 WP_071605322.1 NZ_CP027533:1295029-1295283 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T99171 NZ_CP027533:1295029-1295283 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATTAAACCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12390.89 Da
• Isoelectric Point: 4.4537

>AT99171 WP_063988754.1 NZ_CP027533:1295283-1295624 [Serratia marcescens]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECQKDGIEPYKSYSGKVTTRLSP
ERHQALALAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT99171 NZ_CP027533:1295283-1295624 [Serratia marcescens]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTTGACCCAGAAATTGAAATGTTTCGGGGCGAGTT
TGTCGGGCTGAACGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCAAAAAGACGGCATCGAGCCTTACAAGTCGTACAGTGGCAAAGTAACGACCCGCCTGTCGCCG
GAACGTCATCAGGCGTTGGCTCTTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTAAATGAGGGTGTCGATCT
AGTTATCGAGAAGCACTCTTAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1