Detailed information of TA system TA099161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 286164..286822
• Replicon: chromosome
• Accession: NZ_CP027527
• Organism: Magnetospirillum gryphiswaldense strain R3/S1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6F298
• Locus tag: MSR1L_RS01435
• Protein ID: WP_024080518.1
• Coordinates: 286409..286822 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V6F4U0
• Locus tag: MSR1L_RS01430
• Protein ID: WP_024080519.1
• Coordinates: 286164..286412 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSR1L_RS01400	281264..281743	-	480	WP_158497756.1	response regulator	-
MSR1L_RS01405	281694..282074	+	381	WP_144084300.1	hypothetical protein	-
MSR1L_RS01410	282285..282884	-	600	WP_106001308.1	hypothetical protein	-
MSR1L_RS01415	282887..283324	-	438	WP_024080522.1	protein P5	-
MSR1L_RS01420	283388..285688	-	2301	WP_024080521.1	hypothetical protein	-
MSR1L_RS01425	285688..286122	-	435	WP_024080520.1	C40 family peptidase	-
MSR1L_RS01430	286164..286412	+	249	WP_024080519.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MSR1L_RS01435	286409..286822	+	414	WP_024080518.1	type II toxin-antitoxin system VapC family toxin	Toxin
MSR1L_RS01440	286829..287353	-	525	WP_024080517.1	DUF1833 domain-containing protein	-
MSR1L_RS19715	287346..287501	-	156	WP_158699322.1	hypothetical protein	-
MSR1L_RS01450	287676..288086	-	411	WP_106001310.1	hypothetical protein	-
MSR1L_RS19720	288287..288645	-	359	Protein_276	hypothetical protein	-
MSR1L_RS01460	288963..289271	-	309	WP_024080512.1	hypothetical protein	-
MSR1L_RS01465	289268..289471	-	204	WP_106001311.1	helix-turn-helix domain-containing protein	-
MSR1L_RS01470	289678..290367	-	690	WP_041633579.1	hypothetical protein	-
MSR1L_RS01475	290473..291033	+	561	WP_106001312.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	279556..293747	14191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-62)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15137.47 Da
• Isoelectric Point: 4.7582

>T99161 WP_024080518.1 NZ_CP027527:286409-286822 [Magnetospirillum gryphiswaldense]
VSYLVDTCALSELIRPVPAPQVAEWFETVPQEALFISALTLGEIRRGAEKLADGRRRGRIIAWLEIELPDWFESRVLPVD
AAVADEWGRLTARIKQPLPAIDSLIAATALKHRLTVVTRNVADFAAAGVDILNPWEA

Nucleotide

• Length: 414 bp

>T99161 NZ_CP027527:286409-286822 [Magnetospirillum gryphiswaldense]
GTGAGCTATCTGGTCGATACCTGCGCCCTGTCGGAGCTGATCCGGCCCGTCCCGGCGCCACAGGTGGCGGAGTGGTTCGA
GACGGTGCCGCAGGAGGCACTATTCATCAGCGCCCTGACCCTGGGTGAAATCCGCCGGGGTGCCGAGAAACTGGCGGATG
GGCGGCGGCGCGGCCGGATCATCGCCTGGCTGGAGATCGAGTTACCCGACTGGTTCGAGAGCCGCGTGCTGCCGGTCGAT
GCCGCTGTCGCCGACGAATGGGGCCGCCTGACCGCACGGATCAAACAACCGCTGCCAGCCATCGACAGCCTGATCGCCGC
CACCGCCCTGAAGCACCGGCTCACCGTGGTAACCCGCAACGTCGCCGACTTCGCGGCGGCCGGCGTCGATATCCTCAATC
CCTGGGAGGCATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9225.43 Da
• Isoelectric Point: 8.7082

>AT99161 WP_024080519.1 NZ_CP027527:286164-286412 [Magnetospirillum gryphiswaldense]
MHAWQVQDAKARFSELLRSAAAEGPQAITVRGRTTAVVMSQDEYERLKGRKPSLVEFLRSSPLSGVDLDIERDRSPSRNI
EL

Nucleotide

• Length: 249 bp

>AT99161 NZ_CP027527:286164-286412 [Magnetospirillum gryphiswaldense]
ATGCACGCGTGGCAGGTTCAGGACGCCAAGGCCCGGTTCAGCGAGCTGCTGCGCAGCGCCGCAGCCGAAGGCCCGCAGGC
GATCACCGTTCGCGGCCGCACCACGGCGGTGGTGATGTCCCAGGACGAGTACGAACGGCTGAAGGGGCGCAAGCCGTCGC
TGGTGGAATTCCTGCGGTCGTCGCCGCTGTCCGGCGTGGACCTCGATATCGAGCGCGACCGCTCGCCGTCGCGGAACATC
GAGTTGTGA

Structures

Toxin

• AlphaFold DB: V6F298

Antitoxin

• AlphaFold DB: V6F4U0