Detailed information of TA system TA099159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3639426..3639967
• Replicon: chromosome
• Accession: NZ_CP027526
• Organism: Magnetospirillum gryphiswaldense MSR-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MSR1_RS17105
• Protein ID: WP_106003292.1
• Coordinates: 3639426..3639737 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: MSR1_RS17110
• Protein ID: WP_106003293.1
• Coordinates: 3639737..3639967 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSR1_RS17080	3634876..3635601	-	726	WP_024081071.1	ribonuclease PH	-
MSR1_RS17085	3635735..3636787	+	1053	WP_106003870.1	heat-inducible transcriptional repressor HrcA	-
MSR1_RS17090	3636808..3637398	+	591	WP_024081069.1	nucleotide exchange factor GrpE	-
MSR1_RS17095	3637448..3638641	+	1194	WP_158699554.1	PQQ-dependent sugar dehydrogenase	-
MSR1_RS17100	3638611..3639408	-	798	WP_024081067.1	4-hydroxy-tetrahydrodipicolinate reductase	-
MSR1_RS17105	3639426..3639737	-	312	WP_106003292.1	CcdB family protein	Toxin
MSR1_RS17110	3639737..3639967	-	231	WP_106003293.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MSR1_RS17115	3640028..3641176	-	1149	WP_106003294.1	molecular chaperone DnaJ	-
MSR1_RS17120	3641339..3643246	-	1908	WP_106003295.1	molecular chaperone DnaK	-
MSR1_RS17125	3643392..3644303	+	912	WP_106003296.1	DUF1848 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-102)

Antitoxin

• Post-segregation antitoxin CcdA (6-76)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11165.94 Da
• Isoelectric Point: 4.4084

>T99159 WP_106003292.1 NZ_CP027526:c3639737-3639426 [Magnetospirillum gryphiswaldense MSR-1]
MAQFDIYTNPANGMEGGAPFVVDVQSDHLAGLPTRMIVPLAVPDDFQPIRHLNPPMEVGDERFALMTQFMAAIPRQVLGT
PVGSLADRRATILAALDFLFTGI

Nucleotide

• Length: 312 bp

>T99159 NZ_CP027526:c3639737-3639426 [Magnetospirillum gryphiswaldense MSR-1]
ATGGCCCAGTTCGACATCTACACCAATCCGGCCAACGGAATGGAAGGTGGGGCCCCTTTCGTCGTCGATGTGCAATCCGA
CCATCTGGCCGGCCTGCCCACCCGCATGATCGTGCCCCTGGCCGTCCCCGATGATTTCCAGCCCATCCGCCACCTCAACC
CGCCGATGGAGGTGGGTGACGAACGTTTTGCCCTGATGACCCAATTTATGGCCGCCATCCCCCGTCAGGTGCTGGGCACC
CCGGTGGGCAGTCTGGCCGACCGGCGCGCAACCATCCTCGCCGCCCTGGATTTCCTGTTCACCGGCATCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8699.97 Da
• Isoelectric Point: 9.1005

>AT99159 WP_106003293.1 NZ_CP027526:c3639967-3639737 [Magnetospirillum gryphiswaldense MSR-1]
MNAVPKKSCNLSINAELLREAKGLGINLSQTLESELEKLVRQAKAKAWAEENREAIASQTRWMEKHGLLSDHYRFF

Nucleotide

• Length: 231 bp

>AT99159 NZ_CP027526:c3639967-3639737 [Magnetospirillum gryphiswaldense MSR-1]
ATGAACGCAGTGCCGAAAAAGTCCTGCAACCTCAGCATCAATGCCGAGCTCCTGCGCGAGGCCAAGGGCTTGGGCATCAA
CCTGAGCCAGACCCTGGAATCCGAGTTGGAAAAGCTGGTGCGCCAAGCCAAGGCCAAGGCCTGGGCCGAGGAAAACCGCG
AAGCCATCGCCAGCCAGACCCGCTGGATGGAAAAGCACGGTCTGCTGTCCGACCATTACCGGTTCTTCTGA