Detailed information of TA system TA099156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 4206..5314
• Replicon: plasmid unnamed2
• Accession: NZ_CP027519
• Organism: Enterococcus faecium strain AUSMDU00004024

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: C6Y19_RS16245
• Protein ID: WP_000233000.1
• Coordinates: 4445..5314 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: C6Y19_RS16240
• Protein ID: WP_000205227.1
• Coordinates: 4206..4430 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y19_RS16205	1..897	+	897	WP_002326827.1	ParA family protein	-
C6Y19_RS16210	995..1204	+	210	WP_000527318.1	peptide-binding protein	-
C6Y19_RS16215	1222..1494	+	273	WP_002326825.1	antitoxin	-
C6Y19_RS16220	1496..2359	+	864	WP_002332783.1	toxin zeta	-
C6Y19_RS16225	2922..3062	+	141	Protein_4	IS6 family transposase	-
C6Y19_RS16230	3137..3817	+	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
C6Y19_RS16235	3872..4063	+	192	Protein_6	recombinase family protein	-
C6Y19_RS16240	4206..4430	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
C6Y19_RS16245	4445..5314	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
C6Y19_RS16250	5295..6029	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
C6Y19_RS16255	6062..6970	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
C6Y19_RS16260	6967..7447	+	481	Protein_11	GNAT family N-acetyltransferase	-
C6Y19_RS16265	7536..8216	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
C6Y19_RS16270	8425..8637	+	213	WP_002349227.1	hypothetical protein	-
C6Y19_RS16275	8799..9317	+	519	WP_000357244.1	YfbU family protein	-
C6Y19_RS16280	9324..9836	+	513	WP_000774078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / VanHAX / cat(pC221) / erm(B)	-	1..48209	48209
	-	inside	IScluster/Tn	ant(6)-Ia	-	2916..13028	10112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T99156 WP_000233000.1 NZ_CP027519:4445-5314 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T99156 NZ_CP027519:4445-5314 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT99156 WP_000205227.1 NZ_CP027519:4206-4430 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT99156 NZ_CP027519:4206-4430 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1