Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1222..2359 Replicon plasmid unnamed2
Accession NZ_CP027519
Organism Enterococcus faecium strain AUSMDU00004024

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag C6Y19_RS16220 Protein ID WP_002332783.1
Coordinates 1496..2359 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag C6Y19_RS16215 Protein ID WP_002326825.1
Coordinates 1222..1494 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y19_RS16205 1..897 + 897 WP_002326827.1 ParA family protein -
C6Y19_RS16210 995..1204 + 210 WP_000527318.1 peptide-binding protein -
C6Y19_RS16215 1222..1494 + 273 WP_002326825.1 antitoxin Antitoxin
C6Y19_RS16220 1496..2359 + 864 WP_002332783.1 toxin zeta Toxin
C6Y19_RS16225 2922..3062 + 141 Protein_4 IS6 family transposase -
C6Y19_RS16230 3137..3817 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
C6Y19_RS16235 3872..4063 + 192 Protein_6 recombinase family protein -
C6Y19_RS16240 4206..4430 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
C6Y19_RS16245 4445..5314 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein -
C6Y19_RS16250 5295..6029 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
C6Y19_RS16255 6062..6970 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / VanHAX / cat(pC221) / erm(B) - 1..48209 48209
- inside IScluster/Tn ant(6)-Ia - 2916..13028 10112


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T99155 WP_002332783.1 NZ_CP027519:1496-2359 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T99155 NZ_CP027519:1496-2359 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT99155 WP_002326825.1 NZ_CP027519:1222-1494 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT99155 NZ_CP027519:1222-1494 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References