Detailed information of TA system TA099145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 46189..46796
• Replicon: plasmid unnamed2
• Accession: NZ_CP027508
• Organism: Enterococcus faecium strain AUSMDU00004055

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: C6Y17_RS15480
• Protein ID: WP_002321032.1
• Coordinates: 46446..46796 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: C6Y17_RS15475
• Protein ID: WP_002287514.1
• Coordinates: 46189..46452 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y17_RS15425	41225..42124	+	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C6Y17_RS15430	42127..42612	+	486	WP_002296241.1	single-stranded DNA-binding protein	-
C6Y17_RS15435	42978..43238	+	261	WP_002296240.1	hypothetical protein	-
C6Y17_RS15445	43679..43885	+	207	WP_002296239.1	hypothetical protein	-
C6Y17_RS15450	43885..44136	+	252	WP_002296238.1	hypothetical protein	-
C6Y17_RS15455	44151..44588	+	438	WP_002296237.1	hypothetical protein	-
C6Y17_RS15460	44581..45288	+	708	WP_002353391.1	hypothetical protein	-
C6Y17_RS15470	45669..45848	+	180	WP_002305761.1	hypothetical protein	-
C6Y17_RS15475	46189..46452	+	264	WP_002287514.1	hypothetical protein	Antitoxin
C6Y17_RS15480	46446..46796	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C6Y17_RS15485	46820..47434	-	615	WP_010729795.1	recombinase family protein	-
C6Y17_RS15490	47748..48170	+	423	WP_002288787.1	hypothetical protein	-
C6Y17_RS15495	48180..48383	+	204	WP_002299573.1	hypothetical protein	-
C6Y17_RS15500	48586..49179	+	594	Protein_52	recombinase family protein	-
C6Y17_RS15505	49550..50875	+	1326	WP_099098429.1	Y-family DNA polymerase	-
C6Y17_RS15510	50868..51218	+	351	WP_010724479.1	hypothetical protein	-
C6Y17_RS15515	51215..51394	+	180	WP_002300101.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53613	53613
	-	flank	IS/Tn	-	-	48586..48939	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T99145 WP_002321032.1 NZ_CP027508:46446-46796 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T99145 NZ_CP027508:46446-46796 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT99145 WP_002287514.1 NZ_CP027508:46189-46452 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT99145 NZ_CP027508:46189-46452 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0