Detailed information of TA system TA099143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 17132..17651
• Replicon: plasmid unnamed1
• Accession: NZ_CP027507
• Organism: Enterococcus faecium strain AUSMDU00004055

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: C6Y17_RS13950
• Protein ID: WP_000588503.1
• Coordinates: 17394..17651 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: C6Y17_RS13945
• Protein ID: WP_000388479.1
• Coordinates: 17132..17401 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y17_RS13890	12326..13225	+	900	WP_010706442.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C6Y17_RS13895	13228..13713	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
C6Y17_RS13900	13854..14057	-	204	WP_002316074.1	putative holin-like toxin	-
C6Y17_RS13905	14132..14392	+	261	WP_002321813.1	hypothetical protein	-
C6Y17_RS13915	14832..15038	+	207	WP_002296239.1	hypothetical protein	-
C6Y17_RS13920	15038..15289	+	252	WP_002296238.1	hypothetical protein	-
C6Y17_RS13925	15304..15741	+	438	WP_002300851.1	hypothetical protein	-
C6Y17_RS13930	15734..16441	+	708	WP_002300852.1	hypothetical protein	-
C6Y17_RS13940	16822..17001	+	180	WP_001120991.1	hypothetical protein	-
C6Y17_RS13945	17132..17401	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6Y17_RS13950	17394..17651	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
C6Y17_RS13955	18110..19114	+	1005	WP_000455809.1	hypothetical protein	-
C6Y17_RS13960	19279..19893	-	615	WP_001261742.1	recombinase family protein	-
C6Y17_RS13965	20207..20629	+	423	WP_002288787.1	hypothetical protein	-
C6Y17_RS13970	20639..20842	+	204	WP_002299573.1	hypothetical protein	-
C6Y17_RS13975	21053..21637	+	585	WP_002299575.1	recombinase family protein	-
C6Y17_RS13980	21826..22506	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..231024	231024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T99143 WP_000588503.1 NZ_CP027507:17394-17651 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T99143 NZ_CP027507:17394-17651 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT99143 WP_000388479.1 NZ_CP027507:17132-17401 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT99143 NZ_CP027507:17132-17401 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7