Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 45799..46318 Replicon plasmid unnamed2
Accession NZ_CP027503
Organism Enterococcus faecium strain AUSMDU00004142

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag C6Y15_RS16115 Protein ID WP_000588503.1
Coordinates 46061..46318 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag C6Y15_RS16110 Protein ID WP_000388479.1
Coordinates 45799..46068 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y15_RS16055 40993..41892 + 900 WP_010706442.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
C6Y15_RS16060 41895..42380 + 486 WP_002303313.1 single-stranded DNA-binding protein -
C6Y15_RS16065 42521..42724 - 204 WP_002316074.1 putative holin-like toxin -
C6Y15_RS16070 42799..43059 + 261 WP_002321813.1 hypothetical protein -
C6Y15_RS16080 43499..43705 + 207 WP_002296239.1 hypothetical protein -
C6Y15_RS16085 43705..43956 + 252 WP_002296238.1 hypothetical protein -
C6Y15_RS16090 43971..44408 + 438 WP_002300851.1 hypothetical protein -
C6Y15_RS16095 44401..45108 + 708 WP_002300852.1 hypothetical protein -
C6Y15_RS16105 45489..45668 + 180 WP_001120991.1 hypothetical protein -
C6Y15_RS16110 45799..46068 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6Y15_RS16115 46061..46318 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
C6Y15_RS16120 46777..47781 + 1005 WP_000455809.1 hypothetical protein -
C6Y15_RS16125 47946..48560 - 615 WP_001261742.1 recombinase family protein -
C6Y15_RS16130 48874..49296 + 423 WP_002303116.1 hypothetical protein -
C6Y15_RS16135 49306..49509 + 204 WP_002299573.1 hypothetical protein -
C6Y15_RS16140 49711..50304 + 594 Protein_54 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54738 54738
- flank IS/Tn - - 49711..50064 353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T99139 WP_000588503.1 NZ_CP027503:46061-46318 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T99139 NZ_CP027503:46061-46318 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT99139 WP_000388479.1 NZ_CP027503:45799-46068 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT99139 NZ_CP027503:45799-46068 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References