Detailed information of TA system TA099138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1019223..1020331
• Replicon: chromosome
• Accession: NZ_CP027501
• Organism: Enterococcus faecium strain AUSMDU00004142

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: C6Y15_RS05355
• Protein ID: WP_000233000.1
• Coordinates: 1019223..1020092 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: C6Y15_RS05360
• Protein ID: WP_000205227.1
• Coordinates: 1020107..1020331 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y15_RS05330	1015368..1016048	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
C6Y15_RS05335	1016141..1016935	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
C6Y15_RS05340	1017028..1017570	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
C6Y15_RS05345	1017567..1018475	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
C6Y15_RS05350	1018508..1019242	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
C6Y15_RS05355	1019223..1020092	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
C6Y15_RS05360	1020107..1020331	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
C6Y15_RS05365	1020474..1022018	-	1545	WP_002390960.1	recombinase family protein	-
C6Y15_RS05370	1022038..1022454	-	417	WP_000323438.1	recombinase	-
C6Y15_RS05375	1022597..1023277	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
C6Y15_RS05380	1023475..1024080	+	606	WP_000599739.1	cell filamentation protein	-
C6Y15_RS05385	1024096..1024668	+	573	WP_000170424.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	tet(S) / aph(3')-III / ant(6)-Ia	-	1009913..1041856	31943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T99138 WP_000233000.1 NZ_CP027501:c1020092-1019223 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T99138 NZ_CP027501:c1020092-1019223 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT99138 WP_000205227.1 NZ_CP027501:c1020331-1020107 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT99138 NZ_CP027501:c1020331-1020107 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1