99135

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 20890..22027 Replicon plasmid unnamed3
Accession NZ_CP027500
Organism Enterococcus faecium strain AUSMDU00004167

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag C6Y16_RS16380 Protein ID WP_001284311.1
Coordinates 21164..22027 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag C6Y16_RS16375 Protein ID WP_000301765.1
Coordinates 20890..21162 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y16_RS16330 15899..17307 - 1409 Protein_19 plasmid recombination protein -
C6Y16_RS16335 17528..17890 - 363 WP_002298418.1 hypothetical protein -
C6Y16_RS16340 17894..18232 - 339 WP_171970646.1 hypothetical protein -
C6Y16_RS16345 18211..18630 - 420 WP_044384217.1 hypothetical protein -
C6Y16_RS16350 18623..18856 - 234 WP_002303408.1 hypothetical protein -
C6Y16_RS16355 18853..19497 - 645 Protein_24 replication initiation factor domain-containing protein -
C6Y16_RS16360 19573..20253 + 681 WP_002287920.1 IS6-like element IS1216 family transposase -
C6Y16_RS16365 20309..20566 + 258 WP_002347171.1 hypothetical protein -
C6Y16_RS16370 20664..20873 + 210 WP_000527318.1 peptide-binding protein -
C6Y16_RS16375 20890..21162 + 273 WP_000301765.1 antitoxin Antitoxin
C6Y16_RS16380 21164..22027 + 864 WP_001284311.1 toxin zeta Toxin
C6Y16_RS16385 22290..23027 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
C6Y16_RS16390 23152..23235 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
C6Y16_RS16395 23284..23367 - 84 Protein_32 peptide-binding protein -
C6Y16_RS16400 23777..24571 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
C6Y16_RS16405 24664..24915 - 252 Protein_34 GNAT family N-acetyltransferase -
C6Y16_RS16410 25180..26454 + 1275 WP_106913997.1 ISL3-like element ISEfa11 family transposase -
C6Y16_RS16415 26538..26699 - 162 Protein_36 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / VanHAX - 1..46474 46474
- inside IScluster/Tn erm(B) / aph(3')-III / VanHAX - 5147..45853 40706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T99135 WP_001284311.1 NZ_CP027500:21164-22027 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T99135 NZ_CP027500:21164-22027 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT99135 WP_000301765.1 NZ_CP027500:20890-21162 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT99135 NZ_CP027500:20890-21162 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References