Detailed information of TA system TA099132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 18703..19222
• Replicon: plasmid unnamed1
• Accession: NZ_CP027498
• Organism: Enterococcus faecium strain AUSMDU00004167

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: C6Y16_RS14820
• Protein ID: WP_000588503.1
• Coordinates: 18965..19222 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: C6Y16_RS14815
• Protein ID: WP_000388479.1
• Coordinates: 18703..18972 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y16_RS14765	13815..14075	+	261	WP_002295623.1	hypothetical protein	-
C6Y16_RS14775	14512..14718	+	207	WP_002347218.1	hypothetical protein	-
C6Y16_RS14780	14718..14969	+	252	WP_002296238.1	hypothetical protein	-
C6Y16_RS14785	14984..15421	+	438	WP_002300851.1	hypothetical protein	-
C6Y16_RS14790	15414..16121	+	708	WP_002303117.1	hypothetical protein	-
C6Y16_RS14800	16502..16681	+	180	WP_001120991.1	hypothetical protein	-
C6Y16_RS14805	16886..17290	+	405	WP_002287522.1	IS200/IS605-like element ISEfa4 family transposase	-
C6Y16_RS14810	17307..18455	+	1149	WP_002287525.1	IS200/IS605 family element transposase accessory protein TnpB	-
C6Y16_RS14815	18703..18972	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6Y16_RS14820	18965..19222	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
C6Y16_RS16725	19681..19836	+	156	WP_002347142.1	hypothetical protein	-
C6Y16_RS14825	19860..20684	+	825	WP_001809248.1	hypothetical protein	-
C6Y16_RS14830	20849..21463	-	615	WP_001261742.1	recombinase family protein	-
C6Y16_RS14835	21701..22123	+	423	WP_002303116.1	hypothetical protein	-
C6Y16_RS14840	22133..22336	+	204	WP_002299573.1	hypothetical protein	-
C6Y16_RS14845	22547..23131	+	585	WP_002299575.1	recombinase family protein	-
C6Y16_RS14850	23320..24000	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..207001	207001
	-	inside	IScluster/Tn	-	-	16886..24000	7114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T99132 WP_000588503.1 NZ_CP027498:18965-19222 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T99132 NZ_CP027498:18965-19222 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT99132 WP_000388479.1 NZ_CP027498:18703-18972 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT99132 NZ_CP027498:18703-18972 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7