Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47154..47797 Replicon plasmid unnamed
Accession NZ_CP027473
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag AAF93_RS30975 Protein ID WP_001044768.1
Coordinates 47154..47570 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag AAF93_RS30980 Protein ID WP_001261287.1
Coordinates 47567..47797 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS30950 43242..43589 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
AAF93_RS30955 43586..44260 - 675 WP_106907922.1 IS66-like element accessory protein TnpA -
AAF93_RS30960 44314..44700 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
AAF93_RS30965 44828..45733 + 906 WP_106907923.1 alpha/beta hydrolase -
AAF93_RS30970 46447..46992 + 546 WP_001165114.1 hypothetical protein -
AAF93_RS30975 47154..47570 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
AAF93_RS30980 47567..47797 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AAF93_RS31000 48358..48772 + 415 Protein_47 hypothetical protein -
AAF93_RS31005 48774..49556 + 783 WP_032277352.1 site-specific integrase -
AAF93_RS31010 49729..50082 + 354 WP_000864810.1 colicin M immunity protein -
AAF93_RS31015 50090..50281 - 192 Protein_50 lipid II-degrading bacteriocin -
AAF93_RS31020 50285..50428 - 144 Protein_51 transcriptional regulator -
AAF93_RS31025 50495..51934 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / toxB / hlyD / hlyB / hlyA / hlyA / hlyC / espP / espP 1..81624 81624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99128 WP_001044768.1 NZ_CP027473:c47570-47154 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99128 NZ_CP027473:c47570-47154 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99128 WP_001261287.1 NZ_CP027473:c47797-47567 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99128 NZ_CP027473:c47797-47567 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References