Detailed information of TA system TA099118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3025635..3026472
• Replicon: chromosome
• Accession: NZ_CP027472
• Organism: Escherichia coli strain 2014C-3050

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AAF93_RS16865
• Protein ID: WP_000227784.1
• Coordinates: 3025930..3026472 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AAF93_RS16860
• Protein ID: WP_001297137.1
• Coordinates: 3025635..3025946 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAF93_RS16835	3020655..3021602	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AAF93_RS16840	3021624..3023615	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AAF93_RS16845	3023605..3024219	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AAF93_RS16850	3024219..3024548	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AAF93_RS16855	3024560..3025450	+	891	WP_000971336.1	heme o synthase	-
AAF93_RS16860	3025635..3025946	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AAF93_RS16865	3025930..3026472	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AAF93_RS16870	3026528..3027463	-	936	WP_001375746.1	sel1 repeat family protein	-
AAF93_RS16875	3027871..3029235	+	1365	WP_001000977.1	MFS transporter	-
AAF93_RS16880	3029363..3029854	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AAF93_RS16885	3030022..3030933	+	912	WP_106907881.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T99118 WP_000227784.1 NZ_CP027472:3025930-3026472 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99118 NZ_CP027472:3025930-3026472 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99118 WP_001297137.1 NZ_CP027472:3025635-3025946 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99118 NZ_CP027472:3025635-3025946 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3