Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2991557..2992175 Replicon chromosome
Accession NZ_CP027472
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AAF93_RS16690 Protein ID WP_001291435.1
Coordinates 2991957..2992175 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AAF93_RS16685 Protein ID WP_000344800.1
Coordinates 2991557..2991931 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS16675 2986646..2987839 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AAF93_RS16680 2987862..2991011 + 3150 WP_032277507.1 multidrug efflux RND transporter permease subunit -
AAF93_RS16685 2991557..2991931 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AAF93_RS16690 2991957..2992175 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AAF93_RS16695 2992347..2992898 + 552 WP_032277508.1 maltose O-acetyltransferase -
AAF93_RS16700 2993014..2993484 + 471 WP_000136192.1 YlaC family protein -
AAF93_RS16705 2993648..2995198 + 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AAF93_RS16710 2995240..2995593 - 354 WP_032277511.1 DUF1428 family protein -
AAF93_RS16720 2995971..2996282 + 312 WP_000409911.1 MGMT family protein -
AAF93_RS16725 2996313..2996885 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99117 WP_001291435.1 NZ_CP027472:2991957-2992175 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99117 NZ_CP027472:2991957-2992175 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99117 WP_000344800.1 NZ_CP027472:2991557-2991931 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99117 NZ_CP027472:2991557-2991931 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References