Detailed information of TA system TA099115

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2396781..2397625
• Replicon: chromosome
• Accession: NZ_CP027472
• Organism: Escherichia coli strain 2014C-3050

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: AAF93_RS13445
• Protein ID: WP_000854686.1
• Coordinates: 2396781..2397164 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: AAF93_RS13450
• Protein ID: WP_001285602.1
• Coordinates: 2397245..2397625 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAF93_RS13415	2393457..2394161	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
AAF93_RS13420	2394446..2394664	+	219	WP_001040187.1	translation initiation factor IF-1	-
AAF93_RS13430	2395155..2395997	-	843	WP_032278432.1	DUF4942 domain-containing protein	-
AAF93_RS13435	2396082..2396279	-	198	WP_000839253.1	DUF957 domain-containing protein	-
AAF93_RS13440	2396296..2396784	-	489	WP_001054233.1	hypothetical protein	-
AAF93_RS13445	2396781..2397164	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
AAF93_RS13450	2397245..2397625	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AAF93_RS13455	2397636..2398319	-	684	WP_000086768.1	hypothetical protein	-
AAF93_RS13460	2398338..2398559	-	222	WP_000692298.1	DUF987 domain-containing protein	-
AAF93_RS13465	2398622..2399098	-	477	WP_001186726.1	RadC family protein	-
AAF93_RS13470	2399114..2399599	-	486	WP_000214307.1	antirestriction protein	-
AAF93_RS13475	2399691..2400512	-	822	WP_001761104.1	DUF945 domain-containing protein	-
AAF93_RS13485	2400613..2400821	-	209	Protein_2469	DUF905 domain-containing protein	-
AAF93_RS13490	2400922..2401377	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T99115 WP_000854686.1 NZ_CP027472:c2397164-2396781 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T99115 NZ_CP027472:c2397164-2396781 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT99115 WP_001285602.1 NZ_CP027472:c2397625-2397245 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT99115 NZ_CP027472:c2397625-2397245 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG