Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1469784..1470422 Replicon chromosome
Accession NZ_CP027472
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AAF93_RS07780 Protein ID WP_000813794.1
Coordinates 1470246..1470422 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AAF93_RS07775 Protein ID WP_001270286.1
Coordinates 1469784..1470200 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS07755 1464937..1465878 - 942 WP_001251332.1 ABC transporter permease -
AAF93_RS07760 1465879..1466892 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AAF93_RS07765 1466910..1468055 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
AAF93_RS07770 1468300..1469705 - 1406 Protein_1434 PLP-dependent aminotransferase family protein -
AAF93_RS07775 1469784..1470200 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AAF93_RS07780 1470246..1470422 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AAF93_RS07785 1470644..1470874 + 231 WP_000494244.1 YncJ family protein -
AAF93_RS07790 1470966..1472927 - 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AAF93_RS07795 1473000..1473536 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AAF93_RS07800 1473589..1474803 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1474843..1475991 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99112 WP_000813794.1 NZ_CP027472:c1470422-1470246 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99112 NZ_CP027472:c1470422-1470246 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99112 WP_001270286.1 NZ_CP027472:c1470200-1469784 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99112 NZ_CP027472:c1470200-1469784 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References