Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 984350..984821 Replicon chromosome
Accession NZ_CP027472
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag AAF93_RS05100 Protein ID WP_001303511.1
Coordinates 984350..984628 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AAF93_RS05105 Protein ID WP_001302048.1
Coordinates 984630..984821 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS05075 980000..982651 - 2652 WP_000105101.1 exodeoxyribonuclease VIII -
AAF93_RS05080 982750..983025 - 276 WP_001307773.1 hypothetical protein -
AAF93_RS05085 983099..983275 - 177 WP_000245534.1 hypothetical protein -
AAF93_RS05090 983269..983490 - 222 WP_000560218.1 killing protein KilR -
AAF93_RS05095 983911..984063 - 153 WP_001427316.1 DUF1391 family protein -
AAF93_RS05100 984350..984628 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AAF93_RS05105 984630..984821 - 192 WP_001302048.1 hypothetical protein Antitoxin
AAF93_RS05110 984842..985213 - 372 WP_001169687.1 hypothetical protein -
AAF93_RS05115 985311..985613 + 303 WP_000172738.1 transcriptional regulator -
AAF93_RS05120 985610..986035 + 426 WP_000693943.1 Rha family transcriptional regulator -
AAF93_RS05125 986058..987020 + 963 WP_001444941.1 helix-turn-helix domain-containing protein -
AAF93_RS05130 987027..987768 + 742 Protein_949 ATP-binding protein -
AAF93_RS05135 987794..988555 + 762 WP_000450872.1 DUF1627 domain-containing protein -
AAF93_RS05140 988588..988869 + 282 WP_000603384.1 DNA-binding protein -
AAF93_RS05145 988866..989093 + 228 WP_000699809.1 hypothetical protein -
AAF93_RS05150 989086..989397 + 312 WP_001289673.1 hypothetical protein -
AAF93_RS05155 989525..989743 + 219 WP_000683609.1 DUF4014 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 971468..1026898 55430


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T99110 WP_001303511.1 NZ_CP027472:c984628-984350 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T99110 NZ_CP027472:c984628-984350 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT99110 WP_001302048.1 NZ_CP027472:c984821-984630 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT99110 NZ_CP027472:c984821-984630 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References