Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 731094..731925 Replicon chromosome
Accession NZ_CP027472
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag AAF93_RS03745 Protein ID WP_000854814.1
Coordinates 731094..731468 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag AAF93_RS03750 Protein ID WP_001285585.1
Coordinates 731557..731925 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS03705 726490..727656 + 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
AAF93_RS03710 727775..728248 + 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
AAF93_RS03715 728446..729504 + 1059 WP_001200891.1 FUSC family protein -
AAF93_RS03720 729676..730005 + 330 WP_000450409.1 DUF496 family protein -
AAF93_RS03725 730106..730288 - 183 WP_001016348.1 hypothetical protein -
AAF93_RS03730 730360..730488 + 129 Protein_693 transposase domain-containing protein -
AAF93_RS31655 730777..730890 - 114 WP_001161660.1 DUF957 domain-containing protein -
AAF93_RS03740 730903..731097 - 195 WP_000988600.1 hypothetical protein -
AAF93_RS03745 731094..731468 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
AAF93_RS03750 731557..731925 - 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
AAF93_RS03755 731999..732220 - 222 WP_000692323.1 DUF987 domain-containing protein -
AAF93_RS03760 732283..732759 - 477 WP_001186726.1 RadC family protein -
AAF93_RS03765 732775..733254 - 480 WP_000860087.1 antirestriction protein -
AAF93_RS03770 733336..734153 - 818 Protein_701 DUF932 domain-containing protein -
AAF93_RS03780 734253..734486 - 234 WP_001119729.1 DUF905 family protein -
AAF93_RS03785 734565..735020 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T99097 WP_000854814.1 NZ_CP027472:c731468-731094 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T99097 NZ_CP027472:c731468-731094 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT99097 WP_001285585.1 NZ_CP027472:c731925-731557 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT99097 NZ_CP027472:c731925-731557 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References