Detailed information of TA system TA099088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2324965..2325603
• Replicon: chromosome
• Accession: NZ_CP027462
• Organism: Escherichia coli isolate 07-4299

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C6W75_RS12055
• Protein ID: WP_000813794.1
• Coordinates: 2325427..2325603 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6W75_RS12050
• Protein ID: WP_001270286.1
• Coordinates: 2324965..2325381 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W75_RS12030	2320117..2321058	-	942	WP_001251315.1	ABC transporter permease	-
C6W75_RS12035	2321059..2322072	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
C6W75_RS12040	2322090..2323235	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
C6W75_RS12045	2323480..2324886	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
C6W75_RS12050	2324965..2325381	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6W75_RS12055	2325427..2325603	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6W75_RS12060	2325825..2326055	+	231	WP_000494244.1	YncJ family protein	-
C6W75_RS12065	2326147..2328108	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6W75_RS12070	2328181..2328717	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C6W75_RS12075	2328770..2329984	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2330024..2331172	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T99088 WP_000813794.1 NZ_CP027462:c2325603-2325427 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T99088 NZ_CP027462:c2325603-2325427 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT99088 WP_001270286.1 NZ_CP027462:c2325381-2324965 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT99088 NZ_CP027462:c2325381-2324965 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB