Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1190472..1191097 Replicon chromosome
Accession NZ_CP027462
Organism Escherichia coli isolate 07-4299

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag C6W75_RS06235 Protein ID WP_000911329.1
Coordinates 1190699..1191097 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag C6W75_RS06230 Protein ID WP_000450524.1
Coordinates 1190472..1190699 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W75_RS06205 1186274..1186744 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
C6W75_RS06210 1186744..1187316 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
C6W75_RS06215 1187462..1188340 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
C6W75_RS06220 1188357..1189391 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
C6W75_RS06225 1189604..1190317 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
C6W75_RS06230 1190472..1190699 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
C6W75_RS06235 1190699..1191097 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
C6W75_RS06240 1191244..1192107 + 864 WP_001267498.1 neutral zinc metallopeptidase -
C6W75_RS06245 1192122..1194137 + 2016 WP_000829293.1 tRNA cytosine(34) acetyltransferase TmcA -
C6W75_RS06250 1194211..1194909 + 699 WP_000679823.1 esterase -
C6W75_RS06255 1195019..1195219 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T99076 WP_000911329.1 NZ_CP027462:1190699-1191097 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T99076 NZ_CP027462:1190699-1191097 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT99076 WP_000450524.1 NZ_CP027462:1190472-1190699 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT99076 NZ_CP027462:1190472-1190699 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References