Detailed information of TA system TA099070

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4765654..4766489
• Replicon: chromosome
• Accession: NZ_CP027461
• Organism: Escherichia coli strain 95-3322

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C6W78_RS24635
• Protein ID: WP_096037512.1
• Coordinates: 4766112..4766489 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6W78_RS24630
• Protein ID: WP_097476260.1
• Coordinates: 4765654..4766022 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W78_RS24595	4762490..4762945	+	456	WP_000581504.1	hypothetical protein	-
C6W78_RS24600	4763024..4763257	+	234	WP_001119729.1	DUF905 family protein	-
C6W78_RS24610	4763357..4764175	+	819	WP_106873778.1	DUF945 domain-containing protein	-
C6W78_RS24615	4764230..4764715	+	486	WP_000849582.1	antirestriction protein	-
C6W78_RS24620	4764731..4765207	+	477	WP_001186726.1	RadC family protein	-
C6W78_RS24625	4765270..4765491	+	222	WP_000692329.1	DUF987 domain-containing protein	-
C6W78_RS24630	4765654..4766022	+	369	WP_097476260.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W78_RS24635	4766112..4766489	+	378	WP_096037512.1	TA system toxin CbtA family protein	Toxin
C6W78_RS24640	4766486..4766974	+	489	WP_001519085.1	hypothetical protein	-
C6W78_RS24645	4766986..4767183	+	198	WP_000839281.1	DUF957 domain-containing protein	-
C6W78_RS24650	4767280..4767849	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
C6W78_RS24660	4768598..4770136	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14002.94 Da
• Isoelectric Point: 7.3522

>T99070 WP_096037512.1 NZ_CP027461:4766112-4766489 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T99070 NZ_CP027461:4766112-4766489 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTTCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACAGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13475.33 Da
• Isoelectric Point: 6.6290

>AT99070 WP_097476260.1 NZ_CP027461:4765654-4766022 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99070 NZ_CP027461:4765654-4766022 [Escherichia coli]
GTGTCAGACGCACTCTCTGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA