99049

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 177773..178323 Replicon chromosome
Accession NZ_CP027461
Organism Escherichia coli strain 95-3322

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag C6W78_RS00965 Protein ID WP_001160966.1
Coordinates 178009..178323 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C6W78_RS00960 Protein ID WP_000125566.1
Coordinates 177773..178006 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W78_RS00935 172846..173133 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C6W78_RS00940 173192..174523 + 1332 WP_001183207.1 MFS transporter -
C6W78_RS00945 174631..175161 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C6W78_RS00950 175154..177016 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
C6W78_RS00955 177208..177687 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
C6W78_RS00960 177773..178006 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C6W78_RS00965 178009..178323 + 315 WP_001160966.1 CcdB family protein Toxin
C6W78_RS00970 178320..179168 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C6W78_RS00975 179175..179552 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C6W78_RS00980 179555..180376 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C6W78_RS00985 180373..181362 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C6W78_RS00990 181362..182648 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T99049 WP_001160966.1 NZ_CP027461:178009-178323 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T99049 NZ_CP027461:178009-178323 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT99049 WP_000125566.1 NZ_CP027461:177773-178006 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT99049 NZ_CP027461:177773-178006 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References