Detailed information of TA system TA099046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 28469..29289
• Replicon: chromosome
• Accession: NZ_CP027461
• Organism: Escherichia coli strain 95-3322

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: -
• Locus tag: C6W78_RS00175
• Protein ID: WP_042021467.1
• Coordinates: 29032..29289 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: C6W78_RS00170
• Protein ID: WP_001519303.1
• Coordinates: 28469..29020 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W78_RS00145	24164..25477	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
C6W78_RS00150	25489..25767	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
C6W78_RS00155	25764..26885	-	1122	WP_000010833.1	M42 family metallopeptidase	-
C6W78_RS26555	27130..27246	-	117	Protein_29	VOC family protein	-
C6W78_RS00160	27284..27544	-	261	WP_000084876.1	hypothetical protein	-
C6W78_RS00165	27671..28417	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
C6W78_RS00170	28469..29020	-	552	WP_001519303.1	N-acetyltransferase	Antitoxin
C6W78_RS00175	29032..29289	-	258	WP_042021467.1	hypothetical protein	Toxin
C6W78_RS00185	29666..29965	-	300	WP_001765420.1	GNAT family N-acetyltransferase	-
C6W78_RS00190	30028..31269	+	1242	Protein_35	helicase YjhR	-
C6W78_RS00195	31852..32832	-	981	WP_042021471.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6W78_RS00200	32897..34003	-	1107	WP_042021473.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9706.23 Da
• Isoelectric Point: 10.8332

>T99046 WP_042021467.1 NZ_CP027461:c29289-29032 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYQINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T99046 NZ_CP027461:c29289-29032 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCAAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT99046 WP_001519303.1 NZ_CP027461:c29020-28469 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT99046 NZ_CP027461:c29020-28469 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA