Detailed information of TA system TA099045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 31089..31648
• Replicon: plasmid unnamed
• Accession: NZ_CP027460
• Organism: Escherichia coli strain 90-3040

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6W76_RS28390
• Protein ID: WP_032206862.1
• Coordinates: 31373..31648 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6W76_RS28385
• Protein ID: WP_001297026.1
• Coordinates: 31089..31373 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W76_RS28340	26733..26945	+	213	WP_001302189.1	ANR family transcriptional regulator	-
C6W76_RS28345	27190..27651	+	462	WP_047088284.1	thermonuclease family protein	-
C6W76_RS28350	27697..27906	+	210	WP_047088285.1	hemolysin expression modulator Hha	-
C6W76_RS28355	27944..28534	+	591	WP_000766812.1	DUF2726 domain-containing protein	-
C6W76_RS28360	28766..29020	+	255	WP_000083830.1	replication regulatory protein RepA	-
C6W76_RS28370	29256..29330	+	75	WP_001370046.1	RepA leader peptide Tap	-
C6W76_RS28375	29323..30180	+	858	WP_047088286.1	incFII family plasmid replication initiator RepA	-
C6W76_RS28385	31089..31373	+	285	WP_001297026.1	ribbon-helix-helix domain-containing protein	Antitoxin
C6W76_RS28390	31373..31648	+	276	WP_032206862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W76_RS28395	31743..31949	+	207	WP_001105064.1	hypothetical protein	-
C6W76_RS28400	32129..33306	-	1178	Protein_39	IS91 family transposase	-
C6W76_RS28405	33306..33671	-	366	WP_000091308.1	hypothetical protein	-
C6W76_RS28410	33708..34852	+	1145	WP_199851309.1	IS3 family transposase	-
C6W76_RS28415	34893..35264	+	372	Protein_42	chromosome partitioning protein ParB	-
C6W76_RS28420	35403..35576	-	174	Protein_43	DUF4113 domain-containing protein	-
C6W76_RS28425	35533..35697	+	165	Protein_44	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / papC / hlyC / hlyA / hlyB / hlyD	1..74247	74247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10680.34 Da
• Isoelectric Point: 9.5302

>T99045 WP_032206862.1 NZ_CP027460:31373-31648 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENS

Nucleotide

• Length: 276 bp

>T99045 NZ_CP027460:31373-31648 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.95 Da
• Isoelectric Point: 5.6558

>AT99045 WP_001297026.1 NZ_CP027460:31089-31373 [Escherichia coli]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT99045 NZ_CP027460:31089-31373 [Escherichia coli]
ATGCAAATGAAAAACAATACCGTACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA