Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4409776..4410407 Replicon chromosome
Accession NZ_CP027459
Organism Escherichia coli strain 90-3040

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6W76_RS23695 Protein ID WP_001259385.1
Coordinates 4409776..4410051 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W76_RS23700 Protein ID WP_000593555.1
Coordinates 4410048..4410407 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W76_RS23680 4404780..4405847 + 1068 WP_032207298.1 HlyD family secretion protein -
C6W76_RS23685 4405844..4408579 + 2736 WP_032207297.1 ribosome-associated ATPase/putative transporter RbbA -
C6W76_RS23690 4408579..4409703 + 1125 WP_001216257.1 ABC transporter permease -
C6W76_RS23695 4409776..4410051 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W76_RS23700 4410048..4410407 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W76_RS23705 4410527..4410928 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6W76_RS23710 4410934..4411740 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6W76_RS23715 4411737..4412501 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C6W76_RS23720 4412501..4413334 - 834 WP_021539320.1 nickel ABC transporter permease subunit NikC -
C6W76_RS23725 4413331..4414275 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4400380..4410407 10027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T99040 WP_001259385.1 NZ_CP027459:4409776-4410051 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99040 NZ_CP027459:4409776-4410051 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99040 WP_000593555.1 NZ_CP027459:4410048-4410407 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99040 NZ_CP027459:4410048-4410407 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTATGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References