Detailed information of TA system TA099037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2889607..2890444
• Replicon: chromosome
• Accession: NZ_CP027459
• Organism: Escherichia coli strain 90-3040

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C6W76_RS16005
• Protein ID: WP_032208386.1
• Coordinates: 2889902..2890444 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C6W76_RS16000
• Protein ID: WP_001297137.1
• Coordinates: 2889607..2889918 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W76_RS15975	2884627..2885574	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C6W76_RS15980	2885596..2887587	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C6W76_RS15985	2887577..2888191	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C6W76_RS15990	2888191..2888520	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C6W76_RS15995	2888532..2889422	+	891	WP_000971336.1	heme o synthase	-
C6W76_RS16000	2889607..2889918	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C6W76_RS16005	2889902..2890444	+	543	WP_032208386.1	GNAT family N-acetyltransferase	Toxin
C6W76_RS16010	2890500..2891435	-	936	WP_032208388.1	sel1 repeat family protein	-
C6W76_RS16015	2891843..2893207	+	1365	WP_106913946.1	MFS transporter	-
C6W76_RS16020	2893335..2893826	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C6W76_RS16025	2893994..2894905	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19943.14 Da
• Isoelectric Point: 8.0422

>T99037 WP_032208386.1 NZ_CP027459:2889902-2890444 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPRQKQFDCGNPVIDKFVHESLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99037 NZ_CP027459:2889902-2890444 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTAGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACACGAATCACTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99037 WP_001297137.1 NZ_CP027459:2889607-2889918 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99037 NZ_CP027459:2889607-2889918 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3