Detailed information of TA system TA099024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 262499..263124
• Replicon: chromosome
• Accession: NZ_CP027459
• Organism: Escherichia coli strain 90-3040

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6W76_RS01410
• Protein ID: WP_032206177.1
• Coordinates: 262726..263124 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6W76_RS01405
• Protein ID: WP_000450524.1
• Coordinates: 262499..262726 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W76_RS01380	258301..258771	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6W76_RS01385	258771..259343	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6W76_RS01390	259489..260367	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6W76_RS01395	260384..261418	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6W76_RS01400	261631..262344	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6W76_RS01405	262499..262726	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6W76_RS01410	262726..263124	+	399	WP_032206177.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6W76_RS01415	263271..264134	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
C6W76_RS01420	264149..266164	+	2016	WP_032206176.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6W76_RS01425	266238..266936	+	699	WP_000679812.1	esterase	-
C6W76_RS01430	267046..267246	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14852.17 Da
• Isoelectric Point: 9.2216

>T99024 WP_032206177.1 NZ_CP027459:262726-263124 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPGGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99024 NZ_CP027459:262726-263124 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGGTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99024 WP_000450524.1 NZ_CP027459:262499-262726 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99024 NZ_CP027459:262499-262726 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33