Detailed information of TA system TA099016

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4890179..4891014
• Replicon: chromosome
• Accession: NZ_CP027457
• Organism: Escherichia coli strain 88-3493

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E1LX62
• Locus tag: C6W79_RS24985
• Protein ID: WP_000854745.1
• Coordinates: 4890179..4890556 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A479FB04
• Locus tag: C6W79_RS24990
• Protein ID: WP_047651719.1
• Coordinates: 4890646..4891014 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W79_RS24950	4885721..4886659	-	939	WP_001612791.1	glyoxylate/hydroxypyruvate reductase GhrA	-
C6W79_RS24960	4887130..4887971	-	842	Protein_4575	DUF4942 domain-containing protein	-
C6W79_RS24970	4888252..4889271	-	1020	WP_000875212.1	IS110 family transposase	-
C6W79_RS24975	4889485..4889682	-	198	WP_000839242.1	DUF957 domain-containing protein	-
C6W79_RS24980	4889694..4890182	-	489	WP_041124065.1	hypothetical protein	-
C6W79_RS24985	4890179..4890556	-	378	WP_000854745.1	TA system toxin CbtA family protein	Toxin
C6W79_RS24990	4890646..4891014	-	369	WP_047651719.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W79_RS24995	4891094..4891315	-	222	WP_000691818.1	DUF987 domain-containing protein	-
C6W79_RS25000	4891402..4891878	-	477	WP_001186725.1	RadC family protein	-
C6W79_RS25005	4891894..4892379	-	486	WP_047651721.1	antirestriction protein	-
C6W79_RS25010	4892471..4893289	-	819	WP_047651723.1	DUF945 domain-containing protein	-
C6W79_RS25015	4893379..4893654	-	276	WP_077875616.1	DUF905 family protein	-
C6W79_RS25020	4893689..4894144	-	456	WP_047651727.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	4880140..4923822	43682
	-	flank	IS/Tn	-	-	4888252..4889271	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14071.99 Da
• Isoelectric Point: 7.9308

>T99016 WP_000854745.1 NZ_CP027457:c4890556-4890179 [Escherichia coli]
MKTLPDTHVREASRSPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T99016 NZ_CP027457:c4890556-4890179 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCAGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCTC
CCGACTACTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13542.26 Da
• Isoelectric Point: 5.9562

>AT99016 WP_047651719.1 NZ_CP027457:c4891014-4890646 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPSHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99016 NZ_CP027457:c4891014-4890646 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCAGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCAGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E1LX62

Antitoxin

• AlphaFold DB: A0A479FB04